Potential Pathogenic Bacteria in Loose Oil Palm Fruit (LOPF) from Smallholdings in Serian, Sarawak

Oil palm (Elaeis guineensis) is a species of tree cultivated mainly for its oil. Malaysia the second largest producer oil behind Indonesia. During harvesting fruit bunch, some over ripped fruits will get detached from bunch and these loose be contaminated with bacteria soil. Collecting ground normally done manually by hand this may allow transmission soil to workers.
 
 According Table 1, study reported on microbial contents collected three different locations smallholding in Serian Sarawak, Malaysia. The result shows that F3S3 at Kampung Raeh Baru has highest number colonies, 20.4 x 105 CFU mL-1, while lowest colonies was F2S1, which Beradau, Siburan, 2.4 mL-1.
 This aimed isolate identify (LOPF) determine quality affect safety health farm workers. Loose were smallholder farms located Serian, Sarawak. weighted stomached peptone water stomacher bags plated Nutrient agar. 16S rRNA PCR carried out bacterial growing plate. purified DNA product sent Apical Scientific Sdn. Bhd. sequencing. sequences obtained directly compared Genbank through Blastn server. 2 15 identified samples including Chryseobacterium indologenes, Enterobacter hormaechei, Klebsiella pneumonia, quasipneumoniae, sp., Pantoe dispersa, Pseudomonas aeruginosa, mendocina, nitroreducens, Serratia marcescens, Simplicispira metamorpha. Some could pathogenic, hence, it important communicate findings research provide sufficient knowledge farmers about biological hazards associated order them take precaution proper handling fruits.